Scanning electron microscopy scaffolds

Keywords tissue scaffolds, mercury porosimetry, capillary flow porometry, scanning electron microscopy, image analysis... 

Figure 12.4 Binding points between chitosan fibers (a) and scanning electron microscopy (SEM) of resulting interconnected pore spaces in net-shape-nonwoven scaffolds (b).

A method for encapsulation of nerve growth factor in microspheres of a copolymer of lactic and glycolic acids, and incorporation of these into a polyvinyl alcohol coating to produce tissue engineered scaffolds, is presented. Adherence to, and proliferation on, porous collagen microcarriers, and extension of neurites from the cells were examined using scanning electron microscopy. 11 refs. 

The morphology of the scaffolds was observed by scanning electron microscopy (Nova NanoSEM 200, FEI Co.) after the scaffolds had been dried and coated with graphite, using a sputter coater. 

Figure 6.7 (a,b) Scanning electron microscopy images of poly(L-lactic acid) scaffolds without nanoparticles (c,d) Scaffolds containing 25 wt% bioactive glass-ceramic nanoparticles. Reprinted from Refs [13, 77] with permission. 

Figure 4.1 The biomimetic advantages of nanomaterials. (a) The nanostructuied hierarchical self-assembly of bone, (b) Nanophase titanium (top, atomic force microscopy image) and nanocrystalline HA/ helical rosette nanombe (HRN) hydrogel scaffold (bottom, scanning electron microscopy (SEM) image), (c) Schematic illustration of the mechanism by which nanomaterials may be superior to conventional materials for bone regeneration. The bioactive surfaces of nanomaterials mimic those of natural bones to promote greater amounts of protein adsorption and efficiently stimulate more new bone formation than conventional materials. Zhang, L., Webster, T.J., 2009. Nanotechnology and nanomaterials promises for improved tissue regeneration. Nano Today 4, 66-80.

Figure 10.2 Scanning electron microscopy (SEM) images of electrospun nano- and micro-fibrous poly(E-caprolactone) scaffolds demonstrating fiber dimensions of different orders of magnitude (scale bars 5 pm for subset and 50 pm for full images).

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