Coptisine sanguinarine from

Fig. 36.3 Chemical structures of sanguinarine and coptisine and CZE separation of seven alkaloids in an extract of Chelidonium majus L. Peaks (7) sanguinarine, (2) coptisine, (3) chelerythrine, (4) stylopine, (5) chelidtmme, (6) protopine, (7) allocryptopine, and (x) unknown component. Conditions 20 mM phosphate buffer, pH 3.1. Separation capillary 75 pm, i.d. uncoated fused silica, 50 cm in length (40 cm effective length). Applied voltage 16 kV. Light-emitting-diode-induced fluorescence (LED) excitation wavelength 280 nm (Modified from reference [14])...

Lane 1 (with ascending hRf values) chehdonine, sanguinarine, lane 2 coptisine, lane 3 berbeiine, lane 4 herb, sample preparation according to the DAB, lane 5 herb, 20-min back flow, 70 °C, lane 6 leaves, harvested in May, 20-min back flow, 70 °C, lane 7 roots, harvested in September, 20-min back flow, 70 °C, lane 8 milky juice from the rhizome, diluted with ethanol... 

Cytotoxicity tests in rat hepatocytes indicated that ECgg values for alkaloids isolated from celandine were as follows 5 pg/ml for sanguinarine, 8 pg/ml for chelerythrine, 13 pg/ml for coptisine, 100 pg/ml for prototropine, and over 100 pg/ml for chelidonine (ESCOP 2003). 

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