Sample Preparation and Selection of HPLC Operating Conditions

Sample Preparation and Selection of HPLC Operating Conditions 

Each type of HPLC instrument has its own characteristics and operating directions. It is not feasible to describe those here. However, it is appropriate to outline the general approach taken when an HPLC analysis is desired. The following items must be considered  

Selection of type of chromatography (partition, adsorption, ion exchange, gel exclusion). 

---

Sample Preparation and Selection of HPLC Operating Conditions FPLC—A Modification of HPLC 98... 

Prepared samples can be passed through an ion-exchange column attached to an HPLC system to selectively retain metals present in the samples while the remaining sample is eluted from the column. The separation of organometallic or inorganic ions is favoured by ion exchange columns and the operating conditions are different from the analysis of non-metallic compounds particularly for retention and separation of trace elements. 

---