Saluretic Activity in Rats

Excretion of electrolytes is as important as the excretion of water for treatment of peripheral edema 

Adrenalectomized rats treated with DOCA or aldosterone can be utilized to test aldosterone antagonists. Spironolactone has no effect in the absence of a min-eralocorticoid, but reverses in a dose-related manner the effect of DOCA on the Na+/K+ ratio in the urine (Kagawa et al. 1957 Bicking et al. 1965). 

Bicking JB, Mason JW, Woltersdorf OW et al. (1965) Pyrazine diuretics. I. N-amidino-3-amino-6-halopyrazine-carboxamides. J Med Chem 8 638-642 Durst RA, Siggard-Andersen O (1999) Electrochemistry. In Burtis CA, Ashwood ER (eds) Tietz Textbook of Clinical Chemistry, 3rd edn. W.B. Saunders Company, Philadelphia, pp 133-163 

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