Salmain

Salmain, M., Vessieres, A., Butler, I., and Jaouen, G. (1991) N-Succinimidyl (4-pentynoate)hexacarbon yldicobalt A transition-metal carbonyl complex having similar uses to the Bolton-Hunter reagent. Bioconjugate Chem. 2, 13-15. 

Salmain, M., Fischer-Durand, N., Cavalier L., Rudolf, B., Zakrzewski, J., and Jaouen, G. (2002) Transition metal-carbonyl labeling of biotin and avidin for use in solid-phase carbonyl metallo immunoassay (CMIA). Bioconjugate Cbem. 13, 693-698. 

Varenne, A., Salmain, M., Brisson, C., and Jaouen, G. (1992) Transition metal carbonyl labeling of proteins. A novel approach to a solid-phase two-site immunoassay using Fourier transform infrared spectroscopy. Bioconjugate Chem. 3, 471-476. 

M. Hromadova, M. Salmain, N. Fischer-Durand, L. Pospisil, and G. Jaouen, Electrochemical microbead-based immunoassay using an (h5-cyclopentadienyl)tricarbonylmanganese redox marker bound to bovine serum albumin. Langmuir 22, 506-511 (2006). 

Heldt J-M, Fischer-Durand N, Salmain M, Vessiees A, Jaouen G (2004) J Organomet Chem 689 4775... 

Metzler-Nolte N, Salmain M (2008) The bioorganometallic chemistry of ferrocene. In Stepnicka P (ed) Ferrocenes ligands, materials and biomolecules. Wiley, Chicester, pp 499-639... 

Salmain M,Gunn M, Gorfti A, Top SJaouen G (1993) Bioconjugate Chem 4 425... 

Le Bideau F, Salmain M, Top S et al (2001) New and efficient routes to biomolecules substituted with cyclopentadienyltricarbonylrhenium and -technetium derivatives. Chem Eur J 7 2289-2294 Leon A, Rey A, Mallo L et al (2002) Novel mixed ligand technetium complexes as 5-HTia receptor... 

Many proteins have been labeled with organometallic complexes, mostly for analytical purposes. Some of those are mentioned in Section 1.31.5 of this chapter, and the topic has been comprehensively reviewed by Salmain. Ryabov published an earlier review on the topic.The labeling techniques are mostly the same as for organic derivatives, that is, cysteine-selective reactions (maleiimides, acetic acid halogenides), activated acids, aldehydes, or thiocyanates that react with lysines, biotin-(strept)avidine labeling, and others. 

More recently, the method was extended as an analytical tool for the quantification of antiepileptic drugs in patients. By careful choice of the organometallic complex, it has been possible to quantify two or even three different drugs simultaneously and independently. Scheme 41 shows three such organometallic conjugates of different anti-epileptic drugs as an example. The IR bands used for detection are indicated. Also, interesting applications for environmental analytics such as pesticide analysis were reported mainly by Salmain and co-workers. 

Salmain, M. Labeling of Proteins with Organometallic Complexes Strategies and Applications. In Bioorganometallics-, Jaouen, G., Ed. ... 

Salmain, M. Vessieres, A. Organomeiallic Complexes as Tracers in Non-isotopic Immunoassay. In Bioorganometallics Jaouen, G., Ed. Wiley-VCH Weinheim, 2006 pp 263-302. 

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