Sakaguchi reaction

Cole test (1901) for tryptophan, and the Sakaguchi reaction for arginine (1925). 

Bioautography vs. Bacillus subtilis The chromatographic behavior of B, was similar to B, but B l gave a negative Sakaguchi reaction, while B is positive R r... 

All phleomycins showed Rp below 0.80 and gave positive Sakaguchi reaction Ref. 

Fig. VIII-6. Chromatographic separation and identification of peptides in a thermolysin digest of TNP-clupeine YI. The digestion mixture (from 2.27 p,moles of TNP-clupeine YI) was applied to a column (0.9 cm x 150 cm) of CM-cellulose. Peptides were first eluted with the starting buffer (0.1 M acetate buffer, pH 6.0 tubes 1—80), then an exponential gradient elution was applied (81—514). The mixing chamber contained 1000 ml of starting buffer and to the reservoir 0.1 M acetate buffer (pH 6.5) containing 1.0 M NaCl was added. The peptides remaining on the column were eluted with 0.1 M HCl. The flow rate of the effluent was 3.89 ml/tube/18 min. The effluent was analyzed by Sakaguchi colorimetry and spectrophotometry at 340 mp. The color yield (Sakaguchi reaction) was 101 % (tubes 1 to 555) [from Ando and Suzuki, 1967 Suzuki and Ando 1972 (2)]...

IsHii, S. (1) Sakaguchi reaction on arginine-containing peptides (in Japanese). Nippon Kagaku Zasshi (J. Chem. Soc. Japan, Pure Chem. Sect.) 81, 1586—1588 (1960). 

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