S-oligosaccharides

The variance in binding between 0- and S-oligosaccharides is expected to be small, provided that the differences in bond lengths and

[Pg.111]

The HPLC system used for the analysis of PMP-oligosaccharides included a Waters WISP autosampler and an ABI Model 120 HPLC system. ABI s oligosaccharide HPLC kit-PMP was used for the separation of PMP-oligosaccharides. The kit included a C-18 reversed-phase column, buffer A (10% acetonitrile, 10% 1 M ammonium acetate, pH 5.5) and buffer B (25% acetonitrile, 10% 1 M ammonium acetate, pH 5.5). Elution of PMP-oligosaccharides was monitored at UV 245 nm with the use of a PE Nelson Turbochrom data system (Cupertino, CA) for data acquisition and analysis. 

Lou, B, Huynh, H K, Hanessian, S, Oligosaccharide synthesis by remote activation O-protected 3-methoxy-2-pyridyloxy (MOP) glycosyl donors. In Preparative Carbohydrate Chemistry, Hanessian, S, Ed., Marcel Dekker, New York, pp. 413-430, 1997. 

Lou B, Huynh HK, Hanessian S. Oligosaccharide synthesis by remote activation O-protected glycosyl 2-thiopyridylcarbonate donors. In Hanessian S ed., Preparative Carbohydrate Chemistry. New York Marcel Dekker, Inc., 1997 431-448. 

Kunz C, Rudloff S, Baier W, Klein N, Strobel S. Oligosaccharides in human milk structural, functional and metabolic aspects. Ann Rev Nutr 2000 20 699-722. 

Yamamoto, M. Takeuehi. M. Shinaki, S. Oligosaccharide binding to a boroiiic-acid-appended phenanthroline.Cu(I) complex which creates superstructural helicatcs and catenates Tetrahedron 2002. 58 (36), 7251-7258, and cited references therein. 

The identihcation of uteroferrin s oligosaccharide structure was also made possible by... 

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