Ruta graveolens, coumarins

Chalepensin (coumarin) (dissected mechanism) Ruta graveolens, Stauranthus peforatus Photophos. (30)... 

Umbelliferone is the pivotal metabolite in the pathways to substituted coumarins and furano- or pyranocoumarins. Its 7-0-methylation to herniarin (Fig. 4.3) or 0-prenylation and C-alkylation reactions are often observed in coumarin-producing higher plants where the prenylation at C-6 or C-8 mark the entry to the routes branching linear from angular furano- and pyranocoumarins (Fig. 4.5). In Ruta graveolens and Ammi rmjus, the prenylation reactions are catalysed by particulate enzymes (Hamerski et at, 1990), and... 

Orlita, A., Sidwa-Gorycka, M., Paszkiewicz, M. et al. 2008. Application of chitin and chitosan as elicitors of coumarins and fluoroquinolone alkaloids in Ruta graveolens L (Common rue). Biotechnol. Appl. Biochem., 51 91-96. 

Fig. 4. Separation of the furanocoumarin 5- and 8- -methyl-transferases of Ruta graveolens by successive bioelutions from an AH-Sepharose 4B column linked to 5-(3-carboxypro-panamido) xanthotoxin. The second protein fraction of Figure 2 was applied to this column and the irrigant buffer was changed at the column volume (in column units) shown. Enzyme activity was measured using as substrate the coumarin (bergaptol or xanthotoxol) added to the buffer to desorb each enzyme.

Reisch, J., I. NovAk, K. Szendrei, and E. Minker Rutamarin, ein Coumarin-Derivat aus Ruta graveolens. Acta Pharm. Suecica 4, 179 (1967) Chem. Abs. 67, 54057 (1967). 

Steck, W., B. K. Bailey, J. P. Shyluk, and O. L. Gamborg Coumarins and Alkaloids from Cell Cultures of Ruta graveolens. Phytochemistry 10, 191 (1971). 

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