Rosenthal plot

Binding data is more traditionally presented after the manner of Equation 5.12 in the form of a Rosenthal plot (Figure 5.21).25... 

The y-axis in a Rosenthal plot is the ratio of bound (specific binding, fmol/mg) to free (nM) ligand. The x-axis is simply the bound ligand. The line in a Rosenthal plot has a slope of -1 /Kd. The units of KV) are concentration, which are the same units assigned to the free ligand, nM in this example. The x-intercept corresponds to Bmax. 

Rosenthal plot A Rosenthal plot is generated from ligand binding data and is useful for determining maximal specific binding (Bmax). 

Figure 6.5-3. The Rosenthal plot was constructed according to the relationship ...

The reciprocal (Eq. 3) and Scatchard (Eq. 4) plots cannot be applied to experimental data obtained from experiments with whole plasma as it is necessary to know the molecular weight and the amount of protein in the experimental system. However, Sandberg and co-workers (S3) and Rosenthal (R8) have proposed a Scatchard-type plot based on Eq. (5). 

Key A, total ( ) and nonspecific binding (i.e., binding in the presence of 2 nM fluphenazine) (O) was determined in the presence of the indicated concentrations of [3HJ-spiroperidol and B, specifically bound [3H]-spiroperidol (i.e., the difference between total and nonspecific binding) is shown as a function of the concentration of [3H] spiroperidol. Left inset a plot of the data in B according to Rosenthal s method yields an apparent Kd of 0.2 nM and a maximal concentration of specific binding sites of 94.1 fmol/mg protein (this is equivalent to 20.2... 

This modified Scatchard Plot is used if the protein concentration is unknown (Rosenthal 1967). 

Rosenthal s earlier work (R5) with toluene dispersed in water is reported by Treybal (T6) as a plot of HTI/qc vs yielding, for thirteen nozzles... 

The concentration of bound TPT was calculated by subtracting the measured unbound TPT concentration from the known total TPT concentration. TPT-ATI binding was characterized through the construction of Woolf and Rosenthal binding plots, according to the relationships ... 

He found that this relation holds for fibers from a wide variety of polymers, including polypropylene. The data of Hagjer [208] are plotted in Figure 3.51. As seen, Rosenthal s [209] empirical expression fits the data rather well. 

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