RNA-directed fragment libraries

In contrast to microarray-based approaches, sequence-based methods provide a far more precise determination of the transcriptome (104). This new approach is based on next-generation sequencing techniques and is termed RNA-Seq (Figure 5). Methodologically, the first step consists of conversion of RNA into a library of fragmented cDNA. This can be accomplished by either direct fragmentation of RNA or the newly synthesized cDNA. Next, specific adaptors are attached to the cDNA fragments that are amplified by... 

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