Ribozyme catalysis mechanisms study using

Site-specifically modified RNAs have been used in many applications to examine RNA stmcture-function relationships, RNA-protein interactions, RNA-ligand interactions, and RNA-catalysis mechanisms. Some earlier studies demonstrated the use of synthetic oligonucleotides to probe the roles of specific functional groups and detailed mechanisms in ribozyme catalysis (55). The synthesis of nucleoside analogs allows for a full-range of chemical diversity (e.g., inductive effects, space-filling capacity, etc.) to be explored, such that quantitative stmcture activity relationships can be determined for RNA enzymes and other biologically important RNAs (56). 

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