Reversible intravital aggregation of platelets

The test compound is administered 2 h after platelet injection concurrently with the fourth administration of the aggregating agent. Thirty minutes (ADP, PAF, arachidonic acid, thrombin), or 1 h (collagen) after compound administration another control injection of the aggregating agent is given. This injection is either used as an additional control or it may reveal long-term efficacy of a test compound. 

The microcomputer continuously reveals information about aggregation and desegregation of labelled platelets. 

A = counts over thorax B = counts over abdomen Difference A-B Ratio A/B. 

The time course of response is shown in a curve. The area under the curve is calculated by a computer program. 

Statistical significance is calculated using the Student s t-test. 

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