Reversible contact transfer, high-concentration

Protein solubilized in the reverse micellar solution can be transferred back into an aqueous solution by contacting the micellar solution with an aqueous solution containing a high concentration of salt (e.g., KCl, CaCy, which has the capability to exchange with protein in the micelles (Asenjo and Chaudhuri, 1996). Reverse miceUization has been successfully used to separate a variety of proteins including enzymes (e.g., lysozyme, trypsin and ribonucease). 

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