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Reversible addition-fragmentation vectors

In molecular biology, TDT is used to effect the addition of complementary homopolymeric tails to vector and complementary DNA (cDNA) obtained from a mature mRNA template by reverse transcription and PCR amplification. An alternative application of TDT involves labehng of the 3 termini of DNA fragments with a P-labeled dNTP [36], a dideoxynucleoside triphosphate (ddNTP) [37], or a ribonucleoside triphosphate (rNTP) [38]. [Pg.1100]


See other pages where Reversible addition-fragmentation vectors is mentioned: [Pg.206]    [Pg.360]    [Pg.582]    [Pg.348]    [Pg.206]    [Pg.360]    [Pg.206]    [Pg.424]    [Pg.607]    [Pg.175]    [Pg.233]    [Pg.71]    [Pg.83]   
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