Reversibility of protein

In summary, studies carried out with tissue surrogates25 highlight some of the problems that must be overcome before proteins extracted from FFPE tissues can be used for routine proteomic studies. First, these studies demonstrate that reversal of protein-formaldehyde adducts does not assure quantitative extraction of proteins from FFPE tissues or vice-versa. It may ultimately turn out that there is no one universal method that can accomplish both tasks, but that instead, each step will need to be optimized separately. Studies with tissue surrogates also suggest that failure to quantitatively extract the entire protein component from FFPE tissues may result in sampling bias due to the preferential extraction of certain proteins. This behavior may be linked to protein physical properties, such as the isoelectric point. The results of our... 

Denaturation, Reversible, of Proteins, Methods of Study and Interpretation of Data... 

A summary of information on the reversibility of protein titration curves,... 

Early experiments of MacRitchie Alexander (1963) showed that the adsorption behaviour of different proteins can be described by a simple reaction relation given by Eq. (4.80). Important investigation of the reversibility of protein adsorption... 

VIII. THE REVERSIBILITY OF PROTEIN-SURFACTANT COMPLEX FORMATION... 

A number of important questions revolve around the central one of reversibility of protein adsorption. Are proteins, once adsorbed, able to desorb Why are proteins so difficult to remove from a surface What happens in the situation where there is competitive adsorption between different proteins or between proteins and other species Do the different points on an adsorption isotherm correspond to dynamic equilibria If a protein molecule can desorb, does it revert to its original solution configuration and recover its original biological activity Can the Gibbs Adsorption Equation be applied to protein adsorption Some of these questions may be effectively tackled by studies with the film balance. 

MacRitchie, F. 1998. Reversibility of protein adsorption. In Profezns at liquid interfaces, ed. D. Moebius and R. Miller, 149-177. Amsterdam Elsevier Science B.V. 

Willingham W 3rd, Considine RT, Chaney SG, Lee KH, Hall IH (1984) Reversibility of protein synthesis inhibition by quassinoid antineoplastic agents in a rabbit reticuloc5fte... 

B. Datta, D. Chakrabarti, A.L. Roy and N.K. Gupta, Proc. Natl Acad. Sci. US. A., 1988, 85, 3324-3328, Roles of a 67-kDa polypeptide in reversal of protein synthesis inhibition in heme-deficient reticulocyte lysate. 

M. E. McCully, D. A. C. Beck, and V. Daggett, Biochemistry, 47, 7079 (2008). Microscopic Reversibility of Protein Folding in Molecular Dynamics Simulations of the Engrailed... 

Figure 7.8. A representation of the collapse and charge reversal of proteins with change of pH and adsorption of surfactant of opposite charge.

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