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Reverse transcriptase-polymer chain reaction

Figure 6. Amplification of RNA molecules by assays that are sequence- insensitive. The first assay (upper part) combines the polymerase chain reaction (PCR) of DNA templates with reverse transcription and transcription. Commonly used enzymes are TAQ-polym-erase, HIV reverse transcriptase and bacteriophage T7 RNA polymerase. The assay requires a temperature program applying higher temperatures for double strand dissociation. The second assay (lower part) shows the self-sustained sequence replication reaction (3SR) which can be carried out isothermally because double strand dissociation is replaced by enzymatic digestion of the RNA strand in the RNA-DNA duplex. The enzymes used are HIV reverse transcriptase, RNase H and T7 RNA polymerase. Figure 6. Amplification of RNA molecules by assays that are sequence- insensitive. The first assay (upper part) combines the polymerase chain reaction (PCR) of DNA templates with reverse transcription and transcription. Commonly used enzymes are TAQ-polym-erase, HIV reverse transcriptase and bacteriophage T7 RNA polymerase. The assay requires a temperature program applying higher temperatures for double strand dissociation. The second assay (lower part) shows the self-sustained sequence replication reaction (3SR) which can be carried out isothermally because double strand dissociation is replaced by enzymatic digestion of the RNA strand in the RNA-DNA duplex. The enzymes used are HIV reverse transcriptase, RNase H and T7 RNA polymerase.

See other pages where Reverse transcriptase-polymer chain reaction is mentioned: [Pg.961]    [Pg.476]    [Pg.429]    [Pg.961]    [Pg.476]    [Pg.429]    [Pg.114]    [Pg.887]    [Pg.191]    [Pg.191]    [Pg.49]   
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Chain reversal

Chain reversibility

Chain-reaction polymer

Polymer reversibility

Reaction reverse

Reaction reversible

Reactions, reversing

Reverse transcriptase reaction

Reverse transcriptase-polymer chain

Reverse transcriptases Reversible reactions

Reversibility Reversible reactions

Transcriptase

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