Reverse transcriptase free primer

A primer is a very special sequence, which plays an important role in duplication, (i) In RNA, it is a short sequence that is paired with one strand of DNA and provides a free 3 -OH terminus at which a DNA polymerase starts synthesis of a deoxyribonucleotide chain, (ii) In DNA, it is another short sequence, which is complementary to a sequence of messenger RNA and allows reverse transcriptase to start copying the adjacent sequences of mRNA. (iii) In retroviruses, it is a cellular transfer RNA whose elongation initiates RNA-directed DNA synthesis by the DNA polymerase. 

This combination of ribonucleases exceeds the inhibitory capacity of the RNAse inhibitor added to the reverse transcription reactions by more than tenfold. This treatment eliminates RNA (free and hybridized to the cDNA), allowing unfettered access of the cDNA to DNA primers and reducing the possibility of amplification of RNA by Taq polymerases, that may possess low levels of reverse transcriptase activity under some conditions [16, 17],... 

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