Reverse phase chromatography ochratoxin

A Cantafora, M Grossi, M Miraglia, L Benelli. Determination of ochratoxin A in coffee beans using reverse phase high performance liquid chromatography. Riv Soc Ital Sri Alim 12(2) 103-108, 1983. 

V Seidel, E Poglits, K Schiller, W Lindner. Simultaneous determination of ochratoxin A and zearalenone in maize by reversed-phase high-performance liquid chromatography with fluorescence detection and/8-cyclodextrin as mobile phase additive. J Chrom 635 227-235,1993. 

High-performance liquid chromatography (HPLC) remains the most popular method for determination of ochratoxin A. Most HPLC methods use a reversed-phase column and an acidic mobile phase, so the carboxyl group of the mycotoxin is in the undissociated form. The post-column addition of 10% ammonia solution increases the fluorescence emission of ochratoxin A by a factor of 10 (Monaci Palmisano, 2004). 

Jomet, D., Busto, D. O., and Guasch, J. 2000. Solid-phase extraction applied to the determination of ochratoxin A in wines by reversed-phase high-performance liquid chromatography. J. Chromatogr. A. 882 29-35. 

Hernandez, M. J., Moreno, M. G., Duran, E., Guillen, D., and Barroso, C. G. 2006. Validation of two analytical methods for the determination of ochratoxin A hy reversed-phased high-performance liquid chromatography coupled to fluorescence detection in musts and sweet wines from Andalusia. Ana/. Chim. Acta 566 117-121. 

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