Retinol-binding protein assay

Thakkar H, Cornelius J, Dronfield DM, Medcalf EA, Newman DJ, Price CP. Development of a rapid latex enhanced turbidimetric assay for retinol binding protein. Ann Clin Biochem 1991 28 407-11. 

BJ Burn, MA Kutnink. Liquid-chromatographic assay for free and transthyretin-bound retinol-binding protein in semm from normal humans. Clin Chem 35 582-586, 1989. 

BJ Burri, MA Kutnink, TR Neidlinger. Assay of human transthyretin-bound holo-retinol-binding protein with reversed-phase high-performance liquid chromatography. J Chromatogr 567 369-380, 1991. 

Mata, N. L. Phan, K. Han, Y. Assay of retinol-binding protein-transthyretin interaction and techniques to identify competing ligands. Methods Mol. Biol. 2010,652,209-227. 

Separation and Assay. Procedures for the separation, purification, and assay of carotenoids and retinoids by h.p.l.c., g.c., and g.c.-m.s. are given in an extensive article." Another, general, review includes information on the h.p.l.c. separation of retinoids.A particularly useful method has been developed for resolution and analysis of some carotenoid optical isomers.For example, (3R,3 R)-, (3S,3 S)-, and (3/ ,3 5)-astaxanthin were converted into the diastereomeric (-)-camphanic acid diesters, which were separated by h.p.l.c. This procedure has been used to analyse the isomeric composition of a natural astaxanthin sample. An h.p.l.c. procedure for separation of a-, P-, and y-carotenes (173)—(175) and lycopene (176) has been described." Several papers describe methods for the h.p.l.c. separation and purification of various retinal and retinol isomers and derivatives.A procedure for the preparative t.l.c. of oxidation products of retinyl acetate has been described,and a competitive protein-binding radioassay for retinoic has been reported. 

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