Retention volume Reversible cell

On the basis of findings from the preliminary investigation of the above kind in a conventional electrochemical cell, a suitable mobile phase for the reverse phase liquid chromatographic separation of Cu(dtc)2 complexes with electrochemical detection would be 70% acetonitrile-30% water (0.02 M acetate buffer) with NaNOa as supporting electrolyte. Electrodes investigated in the published paper [3] were the same as in the stationary cell and both oxidation and reduction processes for Cu(dtc)2 were compared. A Metrohm ElA 1096 detector cell (wall jet electrode) was used in this particular cell and a Cig reverse phase chromatographic column was employed. Retention volumes of 14.4 and 10.4 ml were obtained for Cu(dedtc)2 and Cu(pydtc)2, respectively. This smaller retention volume of Cu(pydtc)2 may be attributed to the more polar nature of the complexes. Other experimental details are available in reference 3. In addition, the possibility of in situ formation of the Cu(dtc)2 complex as an alternative to ex situ formation of the complex externally to the column also was examined. 

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