Retention mechanisms gradient polymer elution

Sudden-transition gradient polymer elution chromatography is used to obtain separate control of the sorption and precipitation/dissolution retention mechanisms [530,536,537]. In a typical binary gradient the solvent power and polarity of the mo-... 

A cursory review of the macromolecular HPLC literature immediately rewals sharp disagreement concerning even the basis of these separations. For the common case of protein or polymer gradient-elution, a number of different retention mechanisms" or processes have been pro x>sed as relevant ... 

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