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Retention in size-exclusion chromatography

Gel filtration is a size-exclusion chromatography mode, whereby proteins and peptides are resolved according to their hydrodynamic volumes. The most simple and widely accepted approach to describe the retention in size-exclusion chromatography (SEC) is by considering a selective permeation of macromolecules into the pores of the packing on the basis of their molecular size (more precisely, hydrodynamic volume) (see Fig. 1). It is possible to distinguish three cases ... [Pg.384]

See other pages where Retention in size-exclusion chromatography is mentioned: [Pg.279]    [Pg.1005]    [Pg.481]   
See also in sourсe #XX -- [ Pg.269 ]



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