Restriction enzymes storage

Enzymes. Xhol restriction enzyme, calf intestinal alkaline phosphatase, and T4 DNA ligase are available from commercial suppliers complete with reaction buffers. Follow the instructions for use, storage, and shelf-life... 

Enzymes Xhdi, other restriction enzymes, and T4 DNA ligase are supplied with appropriate buffers by most manufacturers. Follow their instructions for storage and shelf-life. 

Figure 4 Comparative gel electrophoresis patterns of fresh (in aqueous glycerol solution) PST 1 Restriction Enzyme produced digest with a Ready-To-Go dried preparation, after 5-month storage at ambient temperature (see Franks et al.) ...

Storage Purified restriction enzymes are generally stored at — 20°C in buffers containing 50% glycerol. Activity loss can be further prevented by adding autoclaved gelatin or BSA to a final concentration of 50—100 fig/ml. Some enzymes (e.g., EcoRI and Pstl) are also stabilized by the presence of neutral detergents (e.g., 0.2% Triton X-100). 

This synthesis of Ap4A (our abbreviation for the compound in reaction (III)) during the course of the usual activation reaction was not restricted to the lysyl synthetase activating en me system but we have found the latter to be a convenient one with which to work, since the enzyme is easily purified, relatively stable over a long period of storage in the frozen state, and inasmuch as its activation does not require the presence of tRNA, although its rate is accelerated by the latter. >... 

Details of enzymes which play a role in food science are restricted in this chapter to only those enzyme properties which are able to provide an insight into the build-up or functionality of enzymes or can contribute to the understanding of enzyme utilization in food analysis or food processing and storage. 

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