Resolution varying with experimental parameters

These observations were made about the pressure spike as the dimensionless speed and load were varied and are in agreement with experimental results presented by Safa et al. (1983). In their experiments, thin film manganln pressure transducers were used in obtaining good resolution of the pressure spike for various operating parameters. 

The simplest form of an HPLC SST involves comparison of the chromatogram with a standard one, allowing comparison of the peak shape and the peak width baseline resolution. Additional parameters that can be experimentally calculated to provide quantitative SST report include the number of theoretical plates, separation factor, resolution, tailing or peak asymmetry factor, accuracy, and precision (RSD of six measurements). Resolution may also be combined with a selectivity test to check the resolution of the analytes from components present in the sample matrix. If matrix components interfere with a method, a matrix blank may be included in the SST. Peak shape and asymmetry, or tailing factor, can have a major impact on the performance of a quantitative method, especially at low amounts or concentrations, and may vary over the lifetime of a column. 

---