Requirements for an Efficient Catalytic Cycle

The first step to develop an enzymatic reactor requires a wide knowledge of enzyme behavior, regarding the substrates and cofactors involved in the catalytic cycle. In whole fungal cultures, these compounds are endogenously produced and their necessary replacement takes place naturally, whereas in enzymatic processes their addition is required to effectively complete the catalytic cycle. Let us consider MnP as a selected example. It has a similar catalytic cycle than other peroxidases. 

When the practical application of the enzyme for the transformation of a substrate is considered, an important goal is to attain the best efficiency, defined as the maximum amount of substrate degraded per minimum units of enzyme inactivated during the reaction. 

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