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Relaxation rates, single molecule magnetism

In many respects, equilibrium physical measurements could be considered most valuable. Nuclear magnetic resonance (NMR) spectra (T, paramagnetic relaxation) were used to probe cooperativity of midazolam with testosterone and a-NF [1388], Atkins and his associates [1389] used a single-molecule fluorescent approach to show allosteric effects of one ligand on the dissociation rate of another substrate, Nile red. Nile red is an allosteric fluorescent substrate and has utility for such studies [1390] evidence could also be obtained for a second binding site [ 13 91 ]. [Pg.603]

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