Relationships between mobile-phase velocity and column efficiency

5 Relationship between mobile-phase velocity and column efficiency 

1 Packed columns. Much has been written on the relationships between linear velocity (or flow rate) and column efficiency. The relationship of most practical utility is the Knox equation (1977) (equation (2.32)), which describes the relationship between the reduced plate height (A) and the reduced velocity (v)  

TaMe 2.1 Relationship between flow rate (Fy), linear velocity (u), analysis time and column efficiency in liquid chromatography using a conventional packed column (5 pm, 100 x 5 mm i.d.) 

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Relationships between mobile phase velocity and column efficiency, such as the Knox equation (equation (2.32)), are developed by taking into account all the possible contributions to band broadening. Thus the total peak variance (a oiumn) arising from band broadening in a packed column is given by... 

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