Rehydratable gels separation

Table III. Run parameters for a rehydratable gel separated on an MRA Cold Focus apparatus. Gel, 3 % T,3.5 % C, 250 l thick, ambient temperature 23° C, Ampholyte 4 per cent Servalyte pH 3-7 in 10 per cent aqueous glycerol with a distance of 7 cm between electrode wick edges. Catholyte, 1.0 M NaOH, anolyte 1.0 M H3PO4. Time given in minutes.

Figure 3. The effect of separators on ultrathin-layer isoelectric focusing in rehydratable gels. Gels (120 jjim) containing 10% Dextran 35 plus 3% glycerol were rehydrated with 3% Servalyt pH 4-6 plus 5%, glycerol with the indicated concentration of different separators. On the left, a mixture of four marker proteins MYH-horse myoglobin, CAR-carbonic anhydrase, LAC-(3-lactoglobulin,...

Figure 3. A) Hemoglobin standards A, F, S and C, AS and C separated on a five per cent T, pH 6-8 gradient of Pharmalyte. B) Same samples on a similar rehydratable gel. Both A and B were made with the same base reagents at the same time and were bonded to silanized glass plates.

Figure 6. A) Separation of cell extracts 113 and 160 on a three per cent T rehydratable gel with 4 per cent pH 3-10 Servalyte and 7 cm between wick edges. B) Same samples separated on a five per cent T gel.

Figure 7. Comparison of densitometric results on cell extract 160 separations on randomized seven month old rehydratable gels in two different laboratories. The NBS separation was scanned on an LKB laser densitometer and MUSC gel was scanned on a Biomed SL-2D soft laser densitometer.

---