Regulation of Vesicular Transmitter Transporters in Permeabilized Cells and Synaptosomes

3 Regulation of Vesicular Transmitter Transporters in Permeabilized Cells and Synaptosomes 

Transmitter uptake into secretory vesicles is an ATP-dependent process. So far these studies have been restricted to isolated secretory vesicles, where intracellular substances necessary for regulation may be lost during purification. Neuroendocrine cells or synaptosomes permeabilized with both pore-forming toxins can be used to study the regulation of transmitter storage without the necessity of purifying secretory vesicles. 

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