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Reductive amination antibody-enzyme

Enzymes that are glycosylated (i.e., HRP and GO) may be oxidized according to the following method to produce aldehyde groups for reductive amination coupling to an antibody molecule. [Pg.802]

Figure 20.14 Periodate oxidation of HRP creates aldehyde groups on the carbohydrate chains of the enzyme. Reaction with a Fab fragment then may be done using reductive amination to produce a lower-molecular-weight complex than would be obtained using intact IgG antibodies. Figure 20.14 Periodate oxidation of HRP creates aldehyde groups on the carbohydrate chains of the enzyme. Reaction with a Fab fragment then may be done using reductive amination to produce a lower-molecular-weight complex than would be obtained using intact IgG antibodies.
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