Recording the Mossbauer Spectrum

A memory sweep is initialized when the MCA receives a start pulse from the function generator at the trigger input (7). The start pulse is synchronized with the sweep of the reference voltage (Vr) for the Mossbauer drive. It opens the first MCA channel when the source velocity passes through the minimum (cf. Fig. 3.3). After this start trigger, a train of 512 channel advance pulses follows with exact delay times of about 100 ps each. On receiving such a channel advance pulse, the 

Since the actual motion of the Mossbauer drive, as for any frequency transmission system, can show phase shifts relative to the reference signal, the ideal folding point (FP) of the raw data in terms of channel numbers may be displaced from the center at channel number (N — l)/2 (= 255.5 in the example seen earlier). The folding routine must take this into account. Phase shift and FP depend on the settings of the feedback loop in the drive control unit. Therefore, any change of the spectrometer velocity tuning requires the recording of a new calibration spectrum. 

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Layer et al. employed UV visible absorption speetroscopy and Mossbauer speetroscopy to characterize Escherichia coli CYaY as an iron donor for the assembly of [Fc2S2] cluster in the scalfold IscU." In this study, the author provided iron in the form of CyaY-Fe ". At time intervals, formation of iron-sulfur clusters on IscU was monitored by UV-visible absorption spectroscopy from the increase of characteristic absorption bands in the 300-700 nm region (Figure 5.11)." The absorption bands at 320,410, and 456 nm and the shoulder at 510 nm present in the UV-visible spectrum at the end of the reaction are characteristic for 6282] clusters assembled in IscU. The author also provided iron in the form of CyaY- Fe , and recorded the Mossbauer spectrum... 

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