Recombinant solid-phase approach

Coiled coils are interesting as designable cross-linkers because of the well-understood relation between the peptide sequence, the folded and dimerized structure, and the binding affinity. However, as described above, hydrogels based on coiled coils are generally weak, with shear moduli only up to 1 kPa. Furthermore, the use of recombinant protein expression or solid-phase peptide synthesis limits the scale on which these materials can be prepared. However, this approach allows tuning both the cross-Unks as well as the backbone to regulate the mechanical properties and incorporate bioactivity. 

According to [5], they approach zero. Generally speaking, one of the features of the solid-phase oxidation is high E value of macroradical recombination. For instance, E of the second degree recombination of peroxy radicals reaches 50 - 100 kJ/mol [11, pp. 64, 69]. However, it is also known that PO recombination mechanism is superimposed with migration of free valences. Intensification of molecular movements due to plasticization or transition to amorphous systems causes an abrupt decrease of to 10 - 20 kJ/mol [11, p. 64]. These values are typical of transfers in liquids [6, p. 81]. In the present case, oxidation proeeeds in the polymer melt, i.e. molecular movements are unfrozen. Moreover, macroradical... 

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