Recombinant fluorescent chemokine fusion

For production of recombinant fluorescent chemokine fusion proteins Trichoplusia ni 5B1-4 cells (H5 high five cells) are grown at 27 °C in suspension cultures in Insect-Xpress medium in 1800 mL Fembach culture flasks. Importandy, the cells grow without FCS facilitating purification of the chemokine from the culture supernatant. 

Recombinant fluorescent chemokine fusion proteins are purified from insect cell culture supernatants by sequential chromatography using... 

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