Real-time PCR probes

Real-time PCR probe lpM TaqMan probe stock (5 -FAM-TGTACGTGAGTGGGCATGTAGCAAGAGG-BHQ, where FAM is 6-carboxyfluorescein and BFIQ the Black FI ole Quencher-1 IDT, Coralville, IA). 

There are several general categories of real-time PCR probes, including hydrolysis, hairpin, and simple hybridization probes. These probes contain a complementary sequence that allows the probe to anneal to the accumulating PCR product, but probes can differ in the number and location of the fluorescent reporters. 

The real-time PCR probes belonging to the systems and primers listed in Table 5.4 can be found in Table 5.5. 

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See also in sourсe #XX -- [ ]

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