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Reaction mapping sensitivity matrix

Matrix-assisted laser desorption-ionization ionizes molecules with molecular masses of 100-1,000,000 Da for analysis by MS and provides high sensitivity, high throughput, and simplicity of operation. MALDI combined with enzymatic reactions and protein chemistry can provide very useful information on molecular masses, peptide maps, and primary structure.15 MALDI-TOF MS can quickly and accurately determine unfractionated mixtures at concentrations below 100 fmol per liter. The obtained data are then calibrated with internal standards, monoisotopic masses are assigned for all prominent peaks, and the peptide list thus generated is used to identify the protein by using a suitable database.16... [Pg.698]


See other pages where Reaction mapping sensitivity matrix is mentioned: [Pg.332]    [Pg.313]    [Pg.333]    [Pg.314]    [Pg.264]    [Pg.4567]    [Pg.142]    [Pg.268]    [Pg.548]   
See also in sourсe #XX -- [ Pg.315 ]

See also in sourсe #XX -- [ Pg.315 ]




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