Raffi nose

Raffi nose 6-Chloro-6-deoxy-a-D-f ructof uranoside... 

Membranes in the third group contain pores with diameters between 5 A and 10 A and are intermediate between truly microporous and truly solution-diffusion membranes. For example, nanofiltration membranes are intermediate between ultrafiltration membranes and reverse osmosis membranes. These membranes have high rejections for the di- and trisaccharides sucrose and raffi-nose with molecular diameters of 10-13 A, but freely pass the monosaccharide fructose with a molecular diameter of about 5-6 A. 

The principle of potential-dependent selectivity control of a galactose oxidase membrane (Johnson et al., 1982, 1985) using galactose, raffi-nose, and dihydroxyacetone as substrates has been discussed in Section 2.2.2. The change of the redox potential influences all reactions in the same direction. However, since the activity is different towards different substrates the degree of conversion of a particular substrate can be affected by the applied potential. At first the better substrates, e.g. raffinose or galactose, can be measured and at optimal potential all substrates are detected. 

Figure 5. Release of mono- and oligosaccharides from the glycopeptide in 0.4N NaOH (10). Approximately 20 mg of peptidophosphogalactomannan were treated in the dark in 0.4N NaOH in an atmosphere of for 72 hr. The saccharides were fractionated, after neutralization, on a Bio-Gel P-2 column (3 X 92 cm). Reference substances of glucose, maltose, raffi-nose, and stachyose showed maximum concentrations in Fractions 103, 81, 85, and 79, respectively. Carbohydrate was quantified by the phenol-sulfuric acid assay (29)...

Saccharose (sucrose) is the dominant oligosaccharide. Other disaccharides do not have quantitative importance. Maltose occurs in small amounts in grapes, bananas and guava. Melibiose, raffi-nose and stachyose have also been detected in grapes. 6-Kestose has been identified in ripe bananas. 

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