Radioligand binding studies

Lazareno S, Gharagozloo P, Kuonen D, Popham A, Birdsall NJ. Subtype-selective positive cooperative interactions between brucine analogs and acetylcholine at muscarinic receptors radioligand binding studies. Mol Pharmacol 1998 53 573-589. 

Objectives of Radioligand Binding Studies These include ... 

Pearl S., Herrick-Davis K., Teitler M., Glick S. Radioligand binding study of noribogaine a likely metabolite of ibogaine. Brain Res. 675 342, 1995. 

Radioligand-binding studies have been used to characterize muscarinic... 

Deecher DC, Teitler M, Soderlund DM, Bornmann WG, Kuehne ME, Glick SD. (1992). Mechanisms of action of ibogaine and harmaline congeners based on radioligand binding studies. Brain Res. 571(2) 242-47. 

Limits on the Interpretation of Equilibrium Radioligand Binding Studies. 263... 

Since oocytes are a transient, small-scale expression system, they are not the best suited for radioligand binding studies as (usually) hundreds of oocytes must be injected to harvest sufficient membranes for such measurements. This is both time-consuming and expensive. However, with high affinity and highly specific activity ligands, the expression of binding sites on the membranes of intact oocytes can be readily quantified (see later). 

Radioligand binding studies of the postmortem brains of patients with major depressive disorder who did not receive antidepressants showed significant increases in the number of these receptors compared with patients who had received antidepressants and normal control subjects. 

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