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Rabbits basic principles

The basic principle for making the rabbit monoclonal antibody is the same as for mouse monoclonals. Rabbit fusion partner cells can fuse to rabbit B-cells to create the rabbit hybridoma cells. Hybridomas are then screened to select for clones with... [Pg.8]

The difficulty of labeling small amounts of often scarce antibodies directly with I led to the development of the indirect two-site immunoradiometric assay procedure.In this procedure an antibody to the IgG of the species in which the reagent or primary antibody is raised is labeled with Thus if it is desired to carry out an indirect immunoradiometric assay of growth hormone using as the reagent antiserum a rabbit anti-growth hormone serum, it is necessary to label an antibody to rabbit IgG to carry out an indirect immunoradiometric assay. Using the same basic principle, it is also possible to carry out a two-site indirect immunoradiometric assay. [Pg.352]


See other pages where Rabbits basic principles is mentioned: [Pg.258]    [Pg.23]    [Pg.5467]    [Pg.347]    [Pg.549]    [Pg.169]    [Pg.510]    [Pg.291]    [Pg.184]    [Pg.155]   
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Rabbits

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