Rabbit anti-tubulin antibody

Terminology is important in describing the source and specificity of antibodies used in immunocytochemistry. The species used to generate antibodies are used to differentiate antibodies. An antibody generated in rabbit to the protein mbulin would be a rabbit anti-tubulin antibody. With both mouse and rabbit being used to make monoclonal antibodies, the species of the animal generating the monoclonal antibody must be stated, and not simply monoclonal to mean antibodies produced in mouse. To identify an antibody, use the species of animal where the antibody was generated and not the term monoclonal. 

Fig. 13.8. Effect of paclitaxel on the morphology of C6 cells in culture. Cells were treated with LCM only (C,D) or paclitaxel-LCM (A,B) for 8 hours. The cells were stained with fluorescein-conjugated wheat germ agglutinin (WGA) to reveal the cell surface, and with Texas red-conjugated secondary antibody to rabbit anti-tubulin to reveal the presence of microtubules. Both channels were recorded simultaneously. Panels A and C show the presence of WGA, while B and D reveal the presence of tubulin. (Taken from ref. 532.)...

Figure 2. Cultured pulmonary artery endothelial cells stained for tubulin (red), actin (green) and DNA (blue). The dual immunofluorescence procedure used rabbit anti-actin IgG and mouse anti-alpha tubulin IgG as primary antibodies. The secondary antibodies used were Texas Red-conjugated goat, anti-rabbit IgG and FITC-conjugated goat, anti-mouse IgG. The sample was also stained with the DNA-specific dye Hoechst 33342. Scale bar is equal to 20 microns.

For example, when using a mouse monoclonal antibody against tubulin in rabbit tissue with a secondary goat anti-mouse 488 fluorophore, the blocking step would... 

Figure 4. MC3T3 cells on the active area of the device immediately after mechanical stimulation. Indirect immunofluorescence using primary antibody against actin (Tubulin 3, Thermo Scientific, used at 1 50) and secondary antibody (Chromeo 488 conjugated Coat anti-Rabbit IgG, Active Motif 1 500) (400X, microscope Olympus BX41, Olympus Cell A Imaging Software).

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