Quenching by Halide Ions

The quenching of uranyl luminescence by halide ions in solutions has been investigated by many workers2,201 202). Quantitative results reported in recent papers have been collected in Table 6. The quenching constant decreases in the order 

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The improved brightness should also be discussed in terms of the extent of quenching of the fluorophore by environmental factors. Among Aequorea GFP variants, YFPs are relatively acid sensitive, and uniquely quenched by halide ions, including chloride (CT). Proton (H+) and Cl synergisticaUy affect the charge state of the chromophore of YFP, thereby suppressing its fluorescence. Citrine and Venus show increased resistance to both and Cl [7,8]. 

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