Quantitative precipitin curve, 13-30,

FlG. 9.—Quantitative Precipitin Curves of Mouse Myeloma Serum J558 with Various Dextrans. [Reprinted, with permission, from A. Lundblad, R. Steller, E. A. Rabat, J. W. Hirst, M. C. Weigort, and M. Cohn, Immunochemistry, 9,535-543 (1972), Pergamon Press.]... 

Figure 5. Quantitative precipitin curves of fractions from Lea substance N-l with anti-I sera Ma (A), Step (B), with type XIV antipneumococcal horse serum (C) and with goat anti-Lea (D).

Fig. 4.—Quantitative, Precipitin Curve of Dextran B-1355-S with Con A.309 [The total amount of dextran in the precipitate is also illustrated. Con A, 42 tg of nitrogen. (Reproduced by permission of the Journal of Biological Chemistry.)]...

Fig. 6.—Quantitative, Precipitin Curves of Rat-liver Glycogen with Con A. (O, Potassium hydroxide-extracted glycogen A, trichloroacetic acid-extracted glycogen , water-extracted glycogen. Con A, 48 /xg of nitrogen. After Ref. 369.)...

For some purposes, one s primary interest may not be in the quantitative precipitin curves as such, but one may wish merely to determine the... 

Fig. 6. Quantitative precipitin curves of mouse myeloma proteins with various dextrans. From Cisar et al. ...

Figure 3. Quantitative precipitin curves of (O) anti-ACE, anti-ACWC, and...

Figure 6. Quantitative precipitin curves of five mannan subfractions against homologous anti-whole-cell serum of parent bakers yeast (O) Fraction A (%) Fraction B (l ) Fraction C ( ) Fraction D ( j Fraction E O.D., optical density (5).

Figure 16. A Quantitative precipitin curves obtained with various succinylated bovine serum albumin (Su-BSA) and anti-bovine serum albumin. The subscript indicates the number of amino groups modified. B Amount of anti-BSA antibodies precipitated with various Su-BSA at equivalence as a function of amino groups succinylated., Monomer x, aggregate. From Habeeb (1967a).

Water-soluble 0-glycosyl derivatives of polyacrylamide have been prepared by co-polymerization of acrylamide and allyl glycosides of various sugars. Quantitative precipitin curves obtained with these co-polymers and lectins were similar to those produced by lectins and naturally occurring polysaccharides thus the co-polymers can serve as model substances for lectin interactions. 

Turbidimetry indicated that precipitate formation depended on the concentration of the protein as well as on that of the polysaccharide, the time-period of incubation, and the ratio of protein to polysaccharide.121,359,360 Precipitin curves determined turbidimetrically differed from those determined by analysis of precipitated nitrogen.360 For these reasons, early turbidimetric studies conducted on crude (rather than purified) con A are difficult to interpret. The quantitative precipitin method, wherein increasing amounts of polysaccharide or glycoprotein are added to standard aliquots of con A, and the resulting precipitate is washed, and analyzed for nitrogen, is now considered the method of choice for studying protein-polysaccharide interaction.170,320... 

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