Pyrene-2,7 diboronic acid

To help visualise the trends in the observed stability constants (.Kobs) documented in Table 3, the observed stability constants (.Kobs) of the diboronic acid sensors 140( =3)-145( =g) are reported in Figure 30 divided by (i.e. relative to) the observed stability constants (. fobs) of their equivalent monoboronic acid analogue 146(pyrene)- In most cases, the observed stability constants (.Kobs) with diboronic acid sensors 140( =3)-145( =g) are higher than for the monoboronic acid sensor 146(pyrene)-... 

Figure 33 Observed stability constants (Kabs) of the diboronic acid sensors 140fn=3j-145fn—8) and the monoboronic acid reference compound 146(pyrene) (denoted n=ref) with the D-configuration of glucose and its derivatives.

The observed stability constants (.Kobs) for 140( =3) 145( =g) divided by the observed stabihty constants (Kobs) of 146(pyrene) are displayed in Figure 35. Overall the diboronic acid sensors retain their selectivity for D-glucose over the other saccharides. 

The observed stability constants ( obs) of sensor 147(phenanthrene pyrene) (with Xex=299 nm and >,ex=342 nm) were calculated by fitting the emission intensities at 417 nm V5. concentration of saccharide curves and are given in Table 5. The observed stability constants (A obs) for the diboronic acid sensor 147(phenanthrene pyrene) ( ex=299 and 342 um) with D-glucose were enhanced relative to those of the monoboronic acid reference compounds 146(pyrene) and 153(phenanthrene)> while the observed stability constants (Aobs) for the diboronic acid sensor... 

In order to determine the binding stoichiometry of the complexes formed within the binding sites of the diboronic acid based sensors 148(pyrene)-152(2-naphthaiene) and elucidate the benefits gained from allosteric interactions, these systems must be contrasted against analogous monobor-onic acid reference compounds. These reference compounds 146(pyrene), 153(phenanthrene) 15 (2-naphthalene) shall bC diSCUSSCd firSt (Figure 39). 

To help visualize the trends of the observed stability constants (. fobs) in Tables 7 and 8, the stability constants of the diboronic acid sensors 148(pyrene)-152(2-naphthaiene) arc rcportcd in Figurc 40 divided by the stability constants of the analogous monoboronic acid reference compounds... 

Figure 40 Observed stability constants (K hs) of the diboronic acid sensors 148(pyr j-152(2. ap/it/iaiene) divided by the observed stability constants (Kobs) of the corresponding monoboronic acid reference compounds (pyrene) l (phencmthrerte) (2-naphthalerte) iO yield relative ValueS With saccharides. The n-configuration of the monosaccharides was used throughout this evaluation.

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