Pyocyanin system, cozymase inactivation

Inactivation of Cozymase in the Pyocyanin System. The usual apozymase system (page 424) will ferment glucose with production of carbon dioxide for some hours. In the presence of fluorides, phosphorylation of glucose still occurred, and this and oxygen uptake were considerably accelerated by pyocyanin (3 X 10 M) but after these additions... 

The later course of oxygen uptake in the pyocyanin system fell exponentially with time, as did also the rate of cozymase inactivation. This is a reflection of the dependence of the inactivation upon the progress of reactions in w hich cozymase itself plays a necessary part, and whose rate is proportional to the quantity of cozymase available. It can be deduced mathematically and was found experimentally that the total oxygen uptake of such a system is proportional to the quantity of added cozymase. Some 30 moles of oxygen were evolved for each mole of added cozymase. The initial rate of inactivation of cozymase in the reaction mixture of the preceding paragraph was about 3 m/rM per mg. apozymase per hour— which is much more rapid than that of its conversion to coenzyme II (see page 426). 

Inactivation by Apozymase Alone. Inactivation of cozymase was first studied in some detail in the pyocyanin system because it was made evident by the transitory effect of cozymase on oxygen uptake by the system but a modified experimental arrangement showed that the inactivation was brought about by apozymase alone, though with character-... 

Yeast (apozy-mase) (68) Inactivation of cozymase -3 Uptake of oxygen in pyocyanin system 0.1 33... 

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