Purple acid phosphatases resonance Raman

Figure 3. Resonance Raman spectrum of purple acid phosphatase. Protein (5 mM) maintained at 5 C In a glass Dewar and probed with 514.5 nm excitation (within the 560 nm phenolate + Fe(III) CT band, e = 4,000 cm" M The broad, underlying feature from 400-550 cm"1 Is due to Raman scattering from glass. (Reproduced from Ref. 14. Copyright 1987 American Chemical Society.)...

Fig. 1 A, B. Resonance Raman spectra of 5 mM purple (A) and 2.7 mM pink (B) bovine spleen acid phosphatase at pH 5.0 and 5°C. Data were collected with use of 514.5 nm excitation, 100 mW incident power, and 140° back-scattering geometry. The spectra, representing an average of 3 (A) and 8 (A) scans were subjected to a 13-point smoothing procedure. (Adapted from Ref. 42)...

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