Purine-pyrimidine base pairs mismatch

Figure 5-13. Plot showing the accumulated charge as a function of time following a potential step to -350 mV at a series of DNA-modified electrodes immersed in a solution containing 2 mM Fe(CN) and 0.5 pM MB. The thiol-terminated sequence (SI 1-5 -AOT ACA GTC ATC GCG-3 ) was hybridized before electrode deposition to its fully base-paired complement (TA), as well as complements that resulted in all of the possible single-base mismatches. Purine-purine, purine-pyrimidine, and pyiimidine-pyiimidine mismatches were all readily detected. (Adapted from ref. 13.)...

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