Purified allergens, analysis

Other techniques for the bioanalytical characterisation of allergenic proteins and for the elucidation of the tertiary stmcture are nuclear magnetic resonance spectroscopy and x-ray structure analysis, in which separated or purified proteins are used. The two techniques are still the only methods available for a determination of the stmcture of macromolecules such as proteins and nucleic acid on an atomic level, but are usually not utilized in standard food analysis. Both methods have been employed successfully in the elucidation of allergenic proteins [33,34]. 

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