Pseudomonas putida GPol

Smith CA, MR Flyman (2004) Oxidation of methyl tcrt-butyl ether by alkane hydroxylase in dicyclopro-pyUcetone-induced and n-octane-grown Pseudomonas putida Gpol. Appl Environ Microbiol 70 4544-4550. 

Sheu D-S, Lee C-Y (2004) Altering the substrate specificity of polyhydroxyalkanoate synthase 1 derived from Pseudomonas putida GPol by localized semirandom mutagenesis. J Bacteriol 186 4177-4184... 

Ruth K, de Roo G, Egli T, Ren Q (2008) Identification of two acyl-CoA synthetases from Pseudomonas putida GPol one is located at the surface of polyhydroxyalkanoate granules. Biomacromolecules 9 1652-1659... 

Most of the mcl-PHA production strains - with the exception of Pseudomonas putida GPol - accumulate alkanoic mcl-PHA also from unrelated carbon substrates through the fatty acid de novo synthesis pathway. Consequently, glucose may result in polymers containing (R)-3-hydroxy fatty acids with even carbon numbers, e.g., (R)-3-hydroxydecanoate, (R)-3-hydroxyoctanoate, (R)-3-hydroxyhexanoate,... 

Fig. 2 Metabolic routes for mcl-PHA biosynthesis. Pseudomonas putida GPol synthesizes PHA through P-oxidation and P. putida KT2440 synthesizes PHA through fatty add de novo synthesis. Special PHA consisting of 4-hydroxyalkanoate, 5- hydroxyalkanoate, or 6-hydroxyalkanoate can be produced by various bacteria when suitable precursors are supplied. 1 acyl-CoA synthetase, 2 acyl-CoA dehydrogenase, 3 enoyl-CoA hydratase, 4 NAD-dependent (5)-3-hydroxyacyl-CoA dehydrogenase, 5 3-ketoacyl-CoA thiolase, 6 (ItFspecific enoyl-CoA hydratase, 7 NADPH-dependent 3-ketoacyl-CoA reducatase, 8 3-hydroxyacyl-CoA epimerase, 9 mcl-PHA polymerase, 10 acetyl-CoA carboxylase, 11 malonyl-CoA-acyl carrier protein (ACP) tiansacylase, 12 3-keto-ACP synthase, 13 3-keto-ACP reductase, 14 3-hydroxyacyl-ACP dehydratase, 15 enoyl-ACP reductase, 16 acyl-ACP thiolase, 17 (l )-3-hydroxyacyl-ACP-CoA transacylase, 18 mcl-PHA polymerase...

Ren Q, Ruth K, Ihssen J, Thony-Meyer L, Zinn M (2007) A simple in vivo bioprocess for producing enantiomerically pure R-hydroxycarboxylic acids with Pseudomonas putida GPol. J Biotechnol 131 897... 

Buhler, Schmid, and coworkers [36] described the development of a recombinant whole-cell biocatalyst for the direct terminal alkylamino-functionalization of fatty acid methyl esters (e.g., dodecanoic acid methyl ester). The model substrate was dodecanoic acid methyl ester, which was oxidized by an alkane monooxygenase (AlkBGT) from Pseudomonas putida GPol to the corresponding... 

As mentioned above for high cell density fed-batch cultures, the two-step fermentation strategy has often been applied, where the cell division is separated from the PHA accumulation phase. With use of octanoic acid as a substrate, PHA contents of up to 75% (w/w) at a cell concentration of 55 gL" and a volumetric productivity of 0.63 gL h were obtained with P. putida GPol under nitrogen limitation (Kim et al. 2002). With Pseudomonas IPT 046, cell concentrations of up to 50 gL with a PHA content of 63% (w/w) and a volumetric productivity of 0.8 gL" h were reached under phosphate limitation using glucose and fructose as a mixed carbon source (Diniz et al. 2004). When oleic acid was used as a substrate for the cultivation of P. putida KT2442, a cell concentration of 141 gL" with a PHA content of 51% (w/w) and a volumetric productivity of 1.91 gL" h were obtained under phosphate limitation (Lee et al. 2000). 

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