Pseudomonas cruciviae

In 1979, Rama Devi [143] isolated a strain of Pseudomonas cruciviae by enrichment culture on caryophyllene. The major metabolite of this bioconversion was a product hydroxylated at the bridgehead and at the allylic position. The yield, however, was very low. 

A similar enzyme has been purified 26-fold from the bacterium Pseudomonas cruciviae (53). It shows a constant ratio (about three) between activity on S-methyl-L-cysteine and on the sulfoxide through purification. It cleaves a wide range of S-alkyl and S (2-carboxyalkyl) cysteines but not L-cysteine itself (or other amino acids). 

FIGURE 20.131 Biotransformation of (-)-P-caryophyllene (451) by Pseudomonas cruciviae, Diplodia gossypina, and Chaetomium cochliodes. 

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