Protocol of Haab etal

Transfer anhbodies and antigens from glycerol buffer into glycerol-free PBS solution using spin columns (BioSpin P6, Bio-Rad Laboratories, Hercules, CA). 

Array solutions from source plate onto poly-L-lysine coated glass slides. 

Rinse microarrays briefly in 3% nonfat milk in PBS, pH 7.4, containing 0.1% Tween-20. This step is intended to remove unboxmd protein. 

Just prior to use, rinse slides with PBS three times at room temperature (1 min for each rinse). Maintain slides in PBS buffer until incubation with sample. 

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