Proteolytic reaction mixed

Proteolytic Reaction System Contains Mixed Chain Reactions... 

Proteolytic reaction on a protein substrate is a mixed enzymatic reaction with several pathways of chain reactions ended differently, depending on the nature of an enzyme and the structure of the substrates (Fig. 4). At the moment that enzyme and substrates are mixed together, the exact concentration of either... 

Therefore, a proteolytic reaction that is a mixed chain reaction system cannot enter a steady-state phase. Therefore, steady state does not exist in proteolytic reaction systems. 

No other cofactors are required for enzymatic catalysts of this reaction. Amino acid analysis on isolated isoenzymes I and III indicate that the lower molecular weight monomers from I are not proteolytic degradation products of the monomers from III [68], When dimers of I (a ) are mixed with dimers of III ()8y8) an equilibrium concentration of II (afi) is formed. 

The use of a mixed-valent, dinuclear iron site, similar to those in hemerythrin and ribonucleotide reductase,to catalyze a nonredox reaction such as phosphate ester hydrolysis is novel and unexpected for a variant of the familiar oxo(hydroxo)-bridged diiron center. In contrast to the general agreement that exists regarding the spectroscopic and physical properties of the PAPs, their kinetics properties and especially their mechanism of action remain controversial. Much of the disagreement stems from the different pH dependences of the catalytic activity of BSPAP and Uf, which is due to the fact that the former is isolated in a proteolytically activated form while the latter is not. Proteolysis results in a substantial increase in optimal pH in addition to an increase in catalytic activity at the optimal pH. "" Current data suggest that many of the spectroscopic studies described in the literature were performed on a catalytically inactive form of the enzyme. As a result, the roles of the trivalent and divalent metal ions in catalysis and in particular the identity of the nucleophilic hydroxide that directly attacks the phosphate ester remain unresolved. 

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